HAIR SERUM

BIOLOGYNUTRIENTS RESTORATIONHAIR REGROWTH PROTOCOLLe REVE Hair Serum

ANATOMY
HAIR DIFFERENTIATION

HAIR CYCLE

(A) Graphic stages of hair follicle (HF) morphogenesis: hair placode (initiation of HFs pattern formation), immature hair germ, hair peg, and fully developed and differentiated anagen hair by the end of first postnatal resting phase of telogen. Mature HF is built of a reservoir of hair follicle Stem Cells (hfSCs) which is connected above to an associated sebaceous gland (SG), located below the responsive hair germ (HG) with underlying dermal papilla (DP). During the cyclic hair growth, each HF undergoes phases of telogen (quiescence, resting), anagen (activation, regrowth), and catagen(degeneration).  (B) During the growth phase, the hair bulb cells comprise actively proliferative matrix cells which undergo final differentiation creating an inner root sheath (IRS) and hair shaft (HS, protruding out of the skin surface, visible part of HF) layers of the HF mini-organ. HS, hair shaft is composed of Ch, the cuticle of the hair shaft; Co, cortex of hair shaft; Me, medulla; DP, dermal papilla; IRS, inner root sheath contains; He, Henle’s layer; Hu, Huxley’s layer; Ci, cuticle; Cp, companion layer; ORS, outer root sheath.

HAIR STEMCELL

In people who genetically have a lot of DHT receptors, which are the reducers of Testosterone, in hair follicle cells, DHT binds to these DHT receptors and Wnt/β-catenin (a key role in stimulating hair follicle stem cells and hair regeneration.) is inhibited, resulting in hair growth It strongly inhibits hypertonicity. To date, approved hair loss treatment agents (Pinasteride or Dutasteride) or Copper inhibit the formation of DHT by inhibiting the activity of the 5-a-reductase enzyme that causes Testosterone to be reduced to DHT (Dihydrotestosterone). And there is Minoxidil ( Vasodilator ), which improves nutrition by increasing blood flow to the hair follicles to increase blood flow to hair growth.

Wnt/β-catenin signaling pathway

Wnt/β-catenin signaling mechanism. 
(A) In unstimulated cells, β-catenin forms a macromolecular complex with Axin, APC, CK1α, and GSK3 (destruction complex). Phosphorylation by CK1α and GSK3 prepares β-catenin to be ubiquitinated and degraded in the proteasome 26S. 
(B) The interaction of Wnt glycoprotein ligands with Frizzled receptors and LRP5/6 co-receptors recruits DVL to the plasma membrane. Then, GSK3 phosphorylates LRP5/6 and β-catenin is no longer phosphorylated and ubiquitinated because the destruction complex disassembles. The β-catenin accumulated in the cytoplasm is translocated to the nucleus where it displaces the co-repressor Groucho and interacts with TCF to activate gene expression.

HAIR LOSS

  • Hereditary hair loss
  • Age
  • Alopecia areata
  • Cancer treatment
  • Childbirth, illness, or other stressors
  • Hair care
  • Hormonal imbalance : polycystic ovary syndrome (PCOS)
  • Scalp infection
  • Medication
  • Scalp psoriasis
  • Pulling your hair
  • Scarring alopecia
  • Sexually transmitted infection :Syphilis
  • Thyroid disease
  • Too little biotin, iron, protein, or zinc
  • Friction
  • Poison : arsenic, thallium, mercury, lithium and warfarin

유전적으로 Testosterone의 환원체인 DHT 수용체가 모근 세포에 많은 사람은, 이 DHT수용체에  DHT가 결합하면  Wnt/β-catenin(a key role in stimulating hair follicle stem cells and hair regeneration.) 이 억제되어 모발의 성장과 분열을 강력히 억제 합니다.
현재까지 공인된 탈모 치료제(Pinasteride or Dutasteride)나  Copper는 Testosterone 이 DHT(Dihydrotestosterone)로 환원되게하는 5-a- Reductase 효소의 활성을 억제하여 DHT가 생기는 것을 억제합니다. 그리고 모낭의 혈류를 증가시켜 모발의 성장에 혈류공급을 증가시켜 영양을 향상 시키는  Minoxidil ( Vasodilator )이 있습니다.

HAIR LOSS TREATMENT STRATEGY

The hair follicle cycle showing the effects of various cytokines, growth promoters, and growth inhibitors regarding different stage transitions. BDNF, brain-derived neurotrophic factor; BMP-2/4, bone morphogenetic protein-2/4; col 17 A1, collagen-type XVII α 1 chain; DKK, Dickkopf-related protein; FGF5/7/18, fibroblast growth factor 5/7; GDNF, glial cell line-derived neurotrophic factor; HGF, hepatocyte growth factor; IGF-1, insulin-like growth factor-1; IL-1/6, interleukin-6; KGF, keratinocyte growth factor; PG D2/E2/F2a, prostaglandin D2/E2/F2a; SCF, stem cell factor; SHH, sonic hedgehog; TGF, transforming growth factor; TNF-α, tumor necrosis factor α; VDR = vitamin D receptor; WNT, wingless-type integration site.

NUTRIENTS TO HAIR GROWTH


Caffeine:(Topical : 1%, Mesotherapy : up to 0.1%)
Caffeine is an alkaloid methylxanthine and functions as a phosphodiesterase inhibitor, promoting cellular proliferation.  Caffeine counteracts the inhibitory effects of testosterone on hair growth, promotes hair shaft elongation, prolongs anagen duration, and stimulates hair matrix keratinocyte proliferation . Caffeine also downregulates testosterone-induced transforming growth factor (TGF)-β1 expression, a hair growth inhibitor, and increases expression of insulin-like growth factor (IGF)-1, a hair growth promoter. Female hair follicles appear to have a higher sensitivity to caffeine.

Capsaicin : (0.075%, raspberry ketone 0.01%)
Capsaicin, via activation of vanilloid receptor-1 and release of calcitonin gene-related peptide from sensory neurons, upregulates IGF-1 and inhibits TGF-β, which induces apoptosis of keratinocytes through the phosphatidylinositol 3-kinase/Akt pathway
Curcumin (5%. combination with Minoxidil), Garlic Gel, Marine Proteins, Melatonin, Onion Juice, Procyanidin(0.75%~1%), Pumpkin Seed Oil, Rosemary Oil, Saw Palmetto etc.
Morus Bombycis Root Extract. (MBRE)를 주성분으로하는 b-Catenin by Wnt/b-Catenin Signal gene Activator( Caronbio  C3  Shampoo)

Aminoacids: 
N-acetyl-L-cysteine (NAC) : Cysteine plays a central role in hair health; it forms dimers that are oxidized to produce cystine, creating disulfide bridges that provide strength and rigidity between keratin strands.
Lysine is thought to play a role in the absorption of iron.
Methionine is vital for both keratin and procollagen synthesis.
Arginine is protective against the negative effects of hydrogen peroxide on hair proteins and hair surface lipids secondary to oxidative coloring or bleaching.

Vitamins & Minerals

Vitamin A: This vitamin helps to produce a substance called sebum. Sebum is created by the scalp, and it acts as a natural conditioner. It keeps hair from breaking off or drying out and becoming itchy.
Dexpanthenol(Vit B5): is involved in collagen synthesis, one of the major components of hair, reduces sebum secretion, and helps create an environment where hair can grow well. It also strengthens weak hair follicles, helps retain moisture, and prevents hair loss caused by dry scalp.
Biotin(Vit B7): Biotin is a B-complex vitamin and cofactor for carboxylase enzymes involved in fatty acid synthesis, amino acid catabolism, gluconeogenesis, and mitochondrial function in hair root cells
Folate(Vit B9): Folate (or folic acid, the synthetic version of folate) helps renew cells that promote hair growth.
Vitamin C: Vitamin C is an antioxidant, and it helps the absorption of iron into the body.
Vitamin D: Vitamin D receptors are intracellular receptors expressed in hair follicles, essential for normal hair cycle and differentiation of the interfollicular epidermis.
The Vit E: derivatives to inhibit lipid peroxidation may limit hair follicle oxidative stress, thus preventing hair loss.
Copper : (Copper Tripeptides-1, 0.01nM)
Human copper-binding peptide GHK-Cu (glycyl-l-histidyl-l-lysine) is able to up- and down-regulate a significant number of human genes.
1) Tighten loose skin and reverse thinning of aged skin
2) Repair protective skin barrier proteins
3) Improve skin firmness, elasticity, and clarity
4) Reduce fine lines, depth of wrinkles, and improve structure of aged skin
5) Smooth rough skin
6) Reduce photodamage, mottled hyperpigmentation, skin spots and lesions
7) Improve overall skin appearance
8) Stimulate wound healing
9) Protect skin cells from UV radiation
10) Reduce inflammation and free radical damage
11) Increase hair growth and thickness, enlarge hair follicle size, Inhibit 5a-Reductase in Hair follicle.

Zinc: (1% pyrithione zinc)
Mechanisms for zinc-associated hair regrowth include antimicrobial, anti-inflammatory, antioxidant, and anti-5α-reductase activity.
Selenium: Zinc and selenium help to keep hormone levels regulated, making them a good support for healthy hair.
Iron: Too little iron (anemia) can be a major cause of hair loss. This makes iron an important mineral for hair health.

Omega-3 Fatty Acids: Omega-3s are found in the cells on the scalp, and they help nourish hair follicles. They also hydrate the scalp and keep hair shiny and healthy.

Foods to Nourish Hair Growth
Salmon : Vitamin D • Protein • Omega-3 Fatty Acids • Selenium
Yellow Peppers : Folate • Vitamin C • Vitamin E
Sunflower Seeds : Vitamin E•Selenium•Folate
Sweet Potatoes : Vitamin A • Vitamin C
Avocados : EFAs • Folate • Vitamin E
Almonds : Biotin • Vitamin E
Guava : Vitamin C•Folate•Protein
Chia & Flax Seeds : Omega-3 Fatty Acids • Protein • Selenium
Spinach : Vitamin A • Iron • Folate • Vitamin C
Eggs : Omega-3s•Biotin•Protein•Zinc•Iron•Folate
Oysters : Zinc • Protein • Iron • Omega-3s • Calcium • Vitamin C


1) HairStemcell  and Hair Life Cycle


 Hair Folicle Development

2) Stemcell Injection
Adipose tissue stem cells (ASCs)

Hair Stemcell Culture 

 

3) MESO INJECTION

STEMCELL: Mesenchymal stem cell (MSC):
Mesenchymal Stem Cell Conditioned Medium (MSC-CM) 
PRP:  Platelet-rich plasma (PRP) contains no less than six fundamental growth factors, including the fibroblast growth factor (b-FGF), platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), transforming growth factor-β (TGF-β), and insulin-like growth factor-1 (IGF-1) discharged after platelet actuation 

EXOSOME :  Multi Growth factors and Interleukin

PDRN :  Several sessions of mesotherapy in Intra-perifollicular PDRN injections at every other week intervals,
with copper tripeptide, growth factors, d-panthenol, riboflavin, biotin, and zinc sulfate under local anesthesia.

GROWTH FACTORS : EGF,  IGF-1, VEGF, FGF-2, PGF, PGE

NUTRITION :  COPPER, Zinc and Others.

 

4) MECHANICAL STIMULATION
Mesorollering, Stretch, Massage, and Acupuncture

Macrophages are first recruited by chemokines produced by stretch and polarized to M2 phenotype. Growth factors such as HGF and IGF-1, released by M2 macrophages, then activate stem cells and facilitate hair regeneration. A hierarchical control system is revealed, from mechanical and Chemicals

5) INCREASE OXYGEN(BLOOD) SUPPLY
  Minoxidil, Folic Acid, Iron, Omega 3, etc.

6) Laser
1,927-nm Fractionated Thulium Laser + PDRN 

7) Hypnotherapy
Three to eight sessions in 21 patients (9 AT/AU and 12 AA), 57% of patients had significant hair regrowth and 42% patients had total hair regrowth. 

8) Aromatherapy
 Cedarwood, lavender, thyme, and rosemary oils 

MESO- AMPLE LIMITED SPECIFICATION
1. Growth Factors
EGF, Oligopeptide-1  : 0.1ppm ~ 1ppm
IGF-1, Oligopeptide-2 : 0.01ppm~0.1ppm
VEGF, Polypeptide-9 : 0.1ppm~0.5ppm
FGF-2, Polypeptide-1 : 0.05ppm~0.1ppm
FGF 7( keratinocyte growth factor) Polypeptide-3 : 0.01ppm~0.1ppm
Platelet-derived growth factor BB( Polypeptide-59, Platelet-derived growth factor AA( Polypeptide-8)
TGF b1 : Promote Telegen
2. PDRN  : 1000ppm~
3. Peptides
Copper Tripeptides : 10ppm~100ppm
Polypeptide-6(Interleukin-10) : 0.05ppm~0.2ppm
4. Aminoacids
N-acetyl-L-cysteine (NAC)
Methionine
Arginine
Phenylalamine
Tyrosine
5. Vitamins & Minerals
Zinc
Biotin
Dexpanthenol
Vit-D
6. Others
Caffeine : 50ppm~200ppm

Mesoroller Specification
Common Form : 192pin, L: 0.5mm, D: 0.125mm
Professional Form : 240pin~600pin, L: 0.2mm~0.75mm, D: 0.125mm~0.2mm

Shampoo : Caronbio C3 

 

 

 

10 Session Procedure